Cufflinks report, contrast GBM_vs_LM

The samples have the following names, conditions, replicate number and number of reads:

sample_name     condition_replicateID    n_reads
________________________________________________
IT3R17-1GBM     GBM_0                    6199579
IT3R18-9GBM     GBM_1                    3636859
IT3R21-5GBaM    GBM_2                    4981780
IT3R1-wt1LM     LM_0                     5768791
IT3R2-wt2LM     LM_1                     6781877
IT3R3-wt3LM     LM_2                     5539406
IT3R4-wt4LM     LM_3                     6015133





Normalized UCSC genome browser tracks

Tracks normalized to the smallest library
IT3R18-9GBM with 3636859 reads are at:

http://genomics-lab.fleming.gr/cgi-bin/hgTracks?db=mm10&hubUrl=http://genomics-lab.fleming.gr/fleming/ITlab/run298theresa/hub.txt


Differentially expressed genes (DEGs)

A speadsheet with differential expression information for the contrast GBM_vs_LM for all known gense and novel genes identified by cufflinks are at:

http://genomics-lab.fleming.gr/fleming/ITlab/run298theresa/cuffdiff_GBM_vs_LM/gene_exp.diff.xlsx


The number of known and novel DEG up to q-value 0.05 is 9244
The number of known DEG up to q-value 0.05 is 57
The number of known and novel DEG up to q-value 0.01 is 7116
The number of known DEG up to q-value 0.01 is 54



Differentially expressed isoforms

A speadsheet with differential expression information for the contrast GBM_vs_LM for all known isoforms and novel isoforms found by cufflinks including biotype information is at:

http://genomics-lab.fleming.gr/fleming/ITlab/run298theresa/cuffdiff_GBM_vs_LM/isoform_exp.diff.xlsx




Multi-Dimensional Scaling plot

Multi-Dimensional Scaling (MDS) plots comprise a means of visualizing the level of similarity of individual cases of a dataset. MDS uses absolute distance metrics such as the classical Euclidean distance as a similarity measure. MDS serves as a quality control and it can be interpreted as follows: when the distance among samples of the same biological condition in the MDS space is small, this is an indication of high correlation and reproducibility among them. When this distance is larger, this constitutes an indication of low correlation and reproducibility among samples. It can help to identify mislabled samples or to exclude poor samples from further analysis.



MDS plot based on gene expression




Volcano plot

A volcano plot is a scatterplot to give an overview of interesting genes. The log2 fold change is plotted on the x-axis and the negative log10 p-value is plotted on the y-axis. A volcano plot combines the results of a statistical test (aka, p-values) with the magnitude of the change enabling quick visual identification of those genes that display large-magnitude changes that are also statistically significant.




Additional files

All files of the cufflinks/cuffdiff analysis can be found at:

http://genomics-lab.fleming.gr/fleming/ITlab/run298theresa/cuffdiff_GBM_vs_LM

The format of all files is explained at http://cole-trapnell-lab.github.io/cufflinks/cuffdiff/index.html#cuffdiff-output-files.
References and more information can be found at http://cole-trapnell-lab.github.io/cufflinks/papers.




This report was made on Mon Jul 25 14:21:59 2016 with the Cufflinks report generator by Martin Reczko.