Report for Auto_user_IONAS-298-Lexogen_ITlab_160705_IT3R17-30_396

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]9,505.52
‣ Number of Q20 Bases [Mbp]7,490.18
Total Number of Reads86,380,438
Mean Length [bp]110
Longest Read [bp]370

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]2,909.041,909.17
Mean Length [bp]9275
Longest Alignment [bp]276271
Mean Coverage Depth1.07×0.70×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C68%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,519,355
Avg Q17 read length60
50AQ171,034,550

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 132,520,262 89%
Live ISPs131,775,268 99%
Test Fragment ISPs1,988,533 2%
Library ISPs129,786,735 98%
CountPercentage
Library ISPs / Percent Enrichment129,786,735 99%
Filtered: Polyclonal35,324,417 27%
Filtered: Primer dimer114,513 <1%
Filtered: Low quality7,352,678 6%
Final Library Reads87,008,333 67%

Report Information

Analysis Info

Run Name R_2016_07_05_12_53_14_user_IONAS-298-Lexogen_ITlab_160705_IT3R17-30
Run Date 2016-07-05 09:54:58+00:00
Run Flows 520
Project LexoGen_QuantSeq,ITLab_3RNAseq
Sample IT3R20-12GBF
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes IT3R17-22_GallBladder-Male-Female_IT3R23-30_wt-Usp22ko
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-298-Lexogen_ITlab_160705_IT3R17-30_396
Analysis Date 2016-07-05
runID O5ULB

Software Version

Torrent_Suite5.0.4
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.3
ion-dbreports5.0.33-1
ion-gpu5.0.0-1
ion-pipeline5.0.16-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1