Report for Auto_user_IONAS-297-Lexogen_ITlab_160630_IT3R2-16_395

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]9,471.64
‣ Number of Q20 Bases [Mbp]7,527.57
Total Number of Reads87,594,880
Mean Length [bp]108
Longest Read [bp]400

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]2,884.421,948.00
Mean Length [bp]8973
Longest Alignment [bp]286270
Mean Coverage Depth1.06×0.71×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C69%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num758,428
Avg Q17 read length62
50AQ17521,056

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 135,482,921 91%
Live ISPs134,979,511 100%
Test Fragment ISPs965,548 <1%
Library ISPs134,013,963 99%
CountPercentage
Library ISPs / Percent Enrichment134,013,963 100%
Filtered: Polyclonal40,623,100 30%
Filtered: Primer dimer279,128 <1%
Filtered: Low quality6,136,072 5%
Final Library Reads87,916,490 66%

Report Information

Analysis Info

Run Name R_2016_06_30_12_32_12_user_IONAS-297-Lexogen_ITlab_160630_IT3R2-16
Run Date 2016-06-30 09:33:46+00:00
Run Flows 520
Project LexoGen_QuantSeq,ITLab_3RNAseq
Sample IT3R12-wt5
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes IT3R2-8-Liver_IT3R9-16-8weeks_IonTouch
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-297-Lexogen_ITlab_160630_IT3R2-16_395
Analysis Date 2016-06-30
runID A7U2Q

Software Version

Torrent_Suite5.0.4
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.3
ion-dbreports5.0.33-1
ion-gpu5.0.0-1
ion-pipeline5.0.16-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1