Report for Auto_user_IONAS-294-ITlab_160617_ChIP_ITC144-145-154-108_392

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]8,973.19
‣ Number of Q20 Bases [Mbp]7,366.67
Total Number of Reads68,969,967
Mean Length [bp]130
Longest Read [bp]385

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]4,485.073,606.34
Mean Length [bp]11394
Longest Alignment [bp]315311
Mean Coverage Depth1.65×1.32×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C73%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,008,339
Avg Q17 read length69
50AQ17733,341

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 132,977,978 90%
Live ISPs132,256,976 99%
Test Fragment ISPs1,390,407 1%
Library ISPs130,866,569 99%
CountPercentage
Library ISPs / Percent Enrichment130,866,569 99%
Filtered: Polyclonal50,986,544 39%
Filtered: Primer dimer1,541,551 1%
Filtered: Low quality9,347,452 7%
Final Library Reads69,001,238 53%

Report Information

Analysis Info

Run Name R_2016_06_17_12_24_20_user_IONAS-294-ITlab_160617_ChIP_ITC144-145-154-108
Run Date 2016-06-17 09:25:54+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC145-InputP60
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-13YK-FOXA1-ChIPs_ITC144-InputP14-bc4_ITC145-InputP60-bc5_ITC154-Med12P60-bc14_repeat-ITC108-wt1Ser2-bc1
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-294-ITlab_160617_ChIP_ITC144-145-154-108_392
Analysis Date 2016-06-17
runID TA46W

Software Version

Torrent_Suite5.0.4
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.3
ion-dbreports5.0.33-1
ion-gpu5.0.0-1
ion-pipeline5.0.16-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1