Dear Yiota, your alignments and tracks are ready. The tracks are at: http://genomics-lab.fleming.gr/cgi-bin/hgTracks?db=mm10&hubUrl=http://genomics-lab.fleming.gr/fleming/ITlab/run293/hub.txt The tracks are normalized to the second smallest sample: ITC147-E15-2.bam wiht 14766791 reads. The smallest sample ITC149-P0-2.bam with 9389193 reads has not been normalized. The sample sizes are as follows: Sample n_reads ITC146-E15-1.bam 20990269 ITC147-E15-2.bam 14766791 ITC148-P0-1.bam 20852686 ITC149-P0-2.bam 9389193 ITC150-P14-1.bam 16776880 ITC151-P14-2.bam 22482930 ITC152-P60-1.bam 17817439 ITC153-P60-2.bam 16917510 Bam files with the alignments (*.bam) are in the dir: http://genomics-lab.fleming.gr/fleming/ITlab/run293 BW, Martin