Report for Auto_user_IONAS-293-ITlab_160613_CHEF_ChIP_ITC150-153_391

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,628.41
‣ Number of Q20 Bases [Mbp]9,059.96
Total Number of Reads75,373,776
Mean Length [bp]141
Longest Read [bp]393

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]6,502.695,263.52
Mean Length [bp]124104
Longest Alignment [bp]322308
Mean Coverage Depth2.39×1.93×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C73%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,116,346
Avg Q17 read length69
50AQ17816,726

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 122,802,307 83%
Live ISPs122,183,837 99%
Test Fragment ISPs1,242,170 1%
Library ISPs120,941,667 99%
CountPercentage
Library ISPs / Percent Enrichment120,941,667 99%
Filtered: Polyclonal34,363,969 28%
Filtered: Primer dimer2,387,593 2%
Filtered: Low quality9,695,688 8%
Final Library Reads75,405,345 62%

Report Information

Analysis Info

Run Name R_2016_06_14_14_56_45_user_IONAS-293-ITlab_160613_CHEF_ChIP_ITC150-153
Run Date 2016-06-14 11:58:46+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC153-P60-2
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-13YK-FOXA1-ChIPs_ITC150-P14-1-bc10_ITC151-P14-2-bc11_ITC152-P60-1-bc12_ITC153-P60-2-bc13
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-293-ITlab_160613_CHEF_ChIP_ITC150-153_391
Analysis Date 2016-06-14
runID TTU8B

Software Version

Torrent_Suite5.0.4
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.3
ion-dbreports5.0.33-1
ion-gpu5.0.0-1
ion-pipeline5.0.16-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1