Report for Auto_user_IONAS-284-ITlab_160322_ChIP_ITC114-117_380

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]9,499.36
‣ Number of Q20 Bases [Mbp]7,719.51
Total Number of Reads73,121,963
Mean Length [bp]129
Longest Read [bp]388

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]6,250.714,969.58
Mean Length [bp]11394
Longest Alignment [bp]326308
Mean Coverage Depth2.35×1.87×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C72%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num698,752
Avg Q17 read length68
50AQ17504,310

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 136,475,609 92%
Live ISPs135,873,943 100%
Test Fragment ISPs997,233 <1%
Library ISPs134,876,710 99%
CountPercentage
Library ISPs / Percent Enrichment134,876,710 100%
Filtered: Polyclonal52,137,581 39%
Filtered: Primer dimer116,110 <1%
Filtered: Low quality11,133,315 8%
Final Library Reads73,137,715 54%

Report Information

Analysis Info

Run Name R_2016_03_22_12_34_57_user_IONAS-284-ITlab_160322_ChIP_ITC114-117
Run Date 2016-03-22 10:36:31+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC115-wt2Input
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-28NKChIPs_ITC114-wt1Input-bc4_ITC115-wt2Input-bc5_ITC116-SET9KO1Input-bc6_ITC117-SET9KO2Input-bc7
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-284-ITlab_160322_ChIP_ITC114-117_380
Analysis Date 2016-03-22
runID C6UG8

Software Version

Torrent_Suite5.0.4
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.3
ion-dbreports5.0.33-1
ion-gpu5.0.0-1
ion-pipeline5.0.16-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1