Report for Auto_user_IONAS-259-ITlab_151201_ChIP_ITC64-65-74-75_349

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]9,799.40
‣ Number of Q20 Bases [Mbp]7,971.04
Total Number of Reads73,937,281
Mean Length [bp]132
Longest Read [bp]384

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]5,996.524,733.85
Mean Length [bp]11494
Longest Alignment [bp]319302
Mean Coverage Depth2.26×1.78×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C75%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num948,066
Avg Q17 read length71
50AQ17709,466

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 130,379,488 88%
Live ISPs129,374,909 99%
Test Fragment ISPs1,374,034 1%
Library ISPs128,000,875 99%
CountPercentage
Library ISPs / Percent Enrichment128,000,875 99%
Filtered: Polyclonal43,000,000 34%
Filtered: Primer dimer190,473 <1%
Filtered: Low quality10,926,338 9%
Final Library Reads73,957,461 58%

Report Information

Analysis Info

Run Name R_2015_12_01_11_54_33_user_IONAS-259-ITlab_151201_ChIP_ITC64-65-74-75
Run Date 2015-12-01 09:56:34+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC64-wt1PHF8
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_4-KNsamples-RepeatSeq-ION158-197_ITC64-wt1PHF8-bc11_ITC65-wt2PHF8-bc12_ITC74-Set8ko1PHF8-bc5_ITC75-Set8ko2PHF8-bc6
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-259-ITlab_151201_ChIP_ITC64-65-74-75_349
Analysis Date 2015-12-01
runID 74RDN

Software Version

Torrent_Suite5.0.2
hostFW7DQV1
ion-analysis5.0.7-1
ion-chefupdates5.0.1
ion-dbreports5.0.19-1
ion-gpu5.0.0-1
ion-pipeline5.0.12-1
ion-plugins5.0.17-1
ion-protonupdates5.0.2
ion-torrentr5.0.0-1