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Report for Auto_user_IONAS-237-ITlab_151110_CHEF_ChIP_ITC94-95-96-98_328

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,952.80
‣ Number of Q20 Bases [Mbp]9,432.73
Total Number of Reads78,355,799
Mean Length [bp]139
Longest Read [bp]401

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]7,468.646,036.37
Mean Length [bp]122103
Longest Alignment [bp]330315
Mean Coverage Depth2.81×2.27×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C78%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,365,929
Avg Q17 read length73
50AQ171,072,201

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 119,431,461 81%
Live ISPs118,603,070 99%
Test Fragment ISPs1,516,441 1%
Library ISPs117,086,629 99%
CountPercentage
Library ISPs / Percent Enrichment117,086,629 99%
Filtered: Polyclonal30,706,855 26%
Filtered: Primer dimer3,237,803 3%
Filtered: Low quality5,038,065 4%
Final Library Reads78,366,594 67%

Report Information

Analysis Info

Run Name R_2015_11_11_11_39_25_user_IONAS-237-ITlab_151110_CHEF_ChIP_ITC94-95-96-98
Run Date 2015-11-11 09:41:17+00:00
Run Flows 520
Project ITLab_ChIPSeq_CHEF
Sample ITC96-wt1Gold48AT
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_8-KNsamples_ITC94-wt1GoldCELL-bc1_ITC95-wt2GoldCELL-bc2_ITC96-wt1Gold48AT-bc3_ITC98-wt1GoldREINB-bc5_CHEF
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-237-ITlab_151110_CHEF_ChIP_ITC94-95-96-98_328
Analysis Date 2015-11-11
runID M7HHS

Software Version

Torrent_Suite5.0.2
hostFW7DQV1
ion-analysis5.0.7-1
ion-chefupdates5.0.1
ion-dbreports5.0.19-1
ion-gpu5.0.0-1
ion-pipeline5.0.12-1
ion-plugins5.0.17-1
ion-protonupdates5.0.2
ion-torrentr5.0.0-1