Report for Auto_user_IONAS-214-ITlab_150508_MSChIP_ITC90-93_298

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]8,714.09
‣ Number of Q20 Bases [Mbp]7,145.77
Total Number of Reads67,601,121
Mean Length [bp]128
Longest Read [bp]394

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]5,185.304,181.57
Mean Length [bp]11193
Longest Alignment [bp]311301
Mean Coverage Depth1.90×1.53×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C77%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num584,560
Avg Q17 read length71
50AQ17449,545

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 120,513,838 81%
Live ISPs119,269,173 99%
Test Fragment ISPs659,532 <1%
Library ISPs118,609,641 99%
CountPercentage
Library ISPs / Percent Enrichment118,609,641 99%
Filtered: Polyclonal39,638,265 33%
Filtered: Primer dimer186,647 <1%
Filtered: Low quality13,161,593 11%
Final Library Reads67,601,121 57%

Report Information

Analysis Info

Run Name R_2015_08_05_12_38_54_user_IONAS-214-ITlab_150508_MSChIP_ITC90-93
Run Date 2015-08-05 09:40:39+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-214-ITlab_150508_MSChIP_ITC90-93_298
Analysis Date 2015-08-05
runID CX3XH

Software Version

Torrent_Suite4.6
hostFW7DQV1
ion-analysis4.6.11-1
ion-chefupdates4.6.0
ion-dbreports4.6.24-1
ion-gpu4.6.0-1
ion-pipeline4.6.11-1
ion-plugins4.6.18-1
ion-protonupdates4.6.0
ion-torrentr4.6.1-1