Report for Auto_user_ION-199-ITlab_150617_CHEF_ChIP_ITC82-85_279

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]7,746.18
‣ Number of Q20 Bases [Mbp]5,982.13
Total Number of Reads65,545,530
Mean Length [bp]118
Longest Read [bp]388

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]4,848.833,899.483,190.20
Mean Length [bp]1028874
Longest Alignment [bp]305299275
Mean Coverage Depth1.83×1.47×1.20×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C74%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num947,949
Avg Q17 read length67
50AQ17705,187

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 118,839,119 80%
Live ISPs118,475,683 100%
Test Fragment ISPs1,004,899 <1%
Library ISPs117,470,784 99%
CountPercentage
Library ISPs / Percent Enrichment117,470,784 100%
Filtered: Polyclonal32,228,652 27%
Filtered: Primer dimer851,920 <1%
Filtered: Low quality20,188,231 17%
Final Library Reads65,545,530 56%

Report Information

Analysis Info

Run Name R_2015_06_18_15_59_11_user_ION-199-ITlab_150617_CHEF_ChIP_ITC82-85
Run Date 2015-06-18 13:24:26+00:00
Run Flows 520
Project ITLab_ChIPSeq_CHEF
Sample ITC84-Tub1Smyd
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_20-ITsamples_ITC82-Tub1H4K91-bc1_ITC83-Tub2H4K91-bc2_ITC84-Tub1Smyd-bc3_ITC85-Tub2Smyd-bc4_CHEF
Barcode Set IonXpress
Analysis Name Auto_user_ION-199-ITlab_150617_CHEF_ChIP_ITC82-85_279
Analysis Date 2015-06-18
runID TZ7EY

Software Version

Torrent_Suite4.4.3
hostFW7DQV1
ion-analysis4.4.11-1
ion-chefupdates4.4.5
ion-dbreports4.4.38-1
ion-gpu4.4.1-1
ion-pipeline4.4.15-1
ion-plugins4.4.23-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1