Report for Auto_user_ION-160-ITlab_150318_ChIP_ITC54-55-68-69_225

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,394.60
‣ Number of Q20 Bases [Mbp]8,093.50
Total Number of Reads78,960,422
Mean Length [bp]131
Longest Read [bp]382

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]7,420.816,173.264,958.94
Mean Length [bp]11910688
Longest Alignment [bp]311304276
Mean Coverage Depth2.80×2.33×1.87×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C58%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,072,854
Avg Q17 read length52
50AQ17616,920

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 133,946,683 90%
Live ISPs133,887,914 100%
Test Fragment ISPs1,397,691 1%
Library ISPs132,490,223 99%
CountPercentage
Library ISPs / Percent Enrichment132,490,223 100%
Filtered: Polyclonal44,982,207 34%
Filtered: Primer dimer96,172 <1%
Filtered: Low quality10,504,940 8%
Final Library Reads78,960,422 60%

Report Information

Analysis Info

Run Name R_2015_03_18_12_47_52_user_ION-160-ITlab_150318_ChIP_ITC54-55-68-69
Run Date 2015-03-18 10:50:09+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC69-Set8KO2ME1
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_16-KNsamples_ITC54-wt1Input-bc1_ITC55-wt2Input-bc2_ITC68-Set8KO1ME1-bc15_ITC69-Set8KO2ME1-bc16
Barcode Set IonXpress
Analysis Name Auto_user_ION-160-ITlab_150318_ChIP_ITC54-55-68-69_225
Analysis Date 2015-03-18
runID ASPUR

Software Version

Torrent_Suite4.4
hostFW7DQV1
ion-analysis4.4.6-1
ion-chefupdates4.4.3
ion-dbreports4.4.22-1
ion-gpu4.4.1-1
ion-pipeline4.4.9-1
ion-plugins4.4.12-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1