Report for Auto_user_ION-158-ITlab_150317_ChIP_ITC58-59-64-65_223

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]11,316.19
‣ Number of Q20 Bases [Mbp]9,368.39
Total Number of Reads82,870,562
Mean Length [bp]136
Longest Read [bp]395

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]8,229.977,094.035,778.45
Mean Length [bp]12711698
Longest Alignment [bp]333333308
Mean Coverage Depth3.10×2.67×2.18×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C79%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,404,526
Avg Q17 read length74
50AQ171,109,786

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 136,710,512 92%
Live ISPs136,434,680 100%
Test Fragment ISPs1,701,002 1%
Library ISPs134,733,678 99%
CountPercentage
Library ISPs / Percent Enrichment134,733,678 100%
Filtered: Polyclonal45,378,704 34%
Filtered: Primer dimer965,569 <1%
Filtered: Low quality7,537,230 6%
Final Library Reads82,870,562 62%

Report Information

Analysis Info

Run Name R_2015_03_17_12_11_39_user_ION-158-ITlab_150317_ChIP_ITC58-59-64-65_
Run Date 2015-03-17 10:13:52+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC65-wt2PHF8
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_16-KNsamples_ITC58-wt1SET8-bc5_ITC59-wt2SET8-bc6_ITC64-wt1PHF8-bc11_ITC65-wt2PHF8-bc12
Barcode Set IonXpress
Analysis Name Auto_user_ION-158-ITlab_150317_ChIP_ITC58-59-64-65_223
Analysis Date 2015-03-17
runID LKZB7

Software Version

Torrent_Suite4.4
hostFW7DQV1
ion-analysis4.4.6-1
ion-chefupdates4.4.3
ion-dbreports4.4.22-1
ion-gpu4.4.1-1
ion-pipeline4.4.9-1
ion-plugins4.4.12-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1