Report for Auto_user_ION-157-ITlab_150313_ChIP_ITC60-63_222

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]11,977.63
‣ Number of Q20 Bases [Mbp]10,046.26
Total Number of Reads82,617,802
Mean Length [bp]144
Longest Read [bp]394

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]9,621.198,340.026,666.33
Mean Length [bp]136124104
Longest Alignment [bp]340340318
Mean Coverage Depth3.62×3.14×2.51×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C81%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num942,424
Avg Q17 read length76
50AQ17760,257

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 132,714,661 89%
Live ISPs131,959,436 99%
Test Fragment ISPs1,126,348 <1%
Library ISPs130,833,088 99%
CountPercentage
Library ISPs / Percent Enrichment130,833,088 99%
Filtered: Polyclonal42,141,960 32%
Filtered: Primer dimer163,115 <1%
Filtered: Low quality7,316,393 6%
Final Library Reads82,617,802 63%

Report Information

Analysis Info

Run Name R_2015_03_13_12_30_26_user_ION-157-ITlab_150313_ChIP_ITC60-63
Run Date 2015-03-13 10:32:22+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC60-wt1POL2
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_16-KNsamples_ITC60-wt1POL2-bc7_ITC61-wt2POL2-bc8_ITC62-Set8KO1POL2-bc9_ITC63-Set8KO2POL2-bc10
Barcode Set IonXpress
Analysis Name Auto_user_ION-157-ITlab_150313_ChIP_ITC60-63_222
Analysis Date 2015-03-13
runID TIGEK

Software Version

Torrent_Suite4.4
hostFW7DQV1
ion-analysis4.4.6-1
ion-chefupdates4.4.3
ion-dbreports4.4.22-1
ion-gpu4.4.1-1
ion-pipeline4.4.9-1
ion-plugins4.4.12-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1