Report for Auto_user_ION-156-ITlab_150312_ChIP_ITC44-45-48-49_221

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,343.53
‣ Number of Q20 Bases [Mbp]8,870.86
Total Number of Reads74,813,096
Mean Length [bp]138
Longest Read [bp]391

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]7,705.166,752.705,515.49
Mean Length [bp]130120102
Longest Alignment [bp]333324314
Mean Coverage Depth2.90×2.54×2.08×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C82%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num809,746
Avg Q17 read length77
50AQ17664,412

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

block_X0_Y9324block_X1288_Y9324block_X2576_Y9324block_X3864_Y9324block_X5152_Y9324block_X6440_Y9324block_X7728_Y9324block_X9016_Y9324block_X10304_Y9324block_X11592_Y9324block_X12880_Y9324block_X14168_Y9324
block_X0_Y7992block_X1288_Y7992block_X2576_Y7992block_X3864_Y7992block_X5152_Y7992block_X6440_Y7992block_X7728_Y7992block_X9016_Y7992block_X10304_Y7992block_X11592_Y7992block_X12880_Y7992block_X14168_Y7992
block_X0_Y6660block_X1288_Y6660block_X2576_Y6660block_X3864_Y6660block_X5152_Y6660block_X6440_Y6660block_X7728_Y6660block_X9016_Y6660block_X10304_Y6660block_X11592_Y6660block_X12880_Y6660block_X14168_Y6660
block_X0_Y5328block_X1288_Y5328block_X2576_Y5328block_X3864_Y5328block_X5152_Y5328block_X6440_Y5328block_X7728_Y5328block_X9016_Y5328block_X10304_Y5328block_X11592_Y5328block_X12880_Y5328block_X14168_Y5328
block_X0_Y3996block_X1288_Y3996block_X2576_Y3996block_X3864_Y3996block_X5152_Y3996block_X6440_Y3996block_X7728_Y3996block_X9016_Y3996block_X10304_Y3996block_X11592_Y3996block_X12880_Y3996block_X14168_Y3996
block_X0_Y2664block_X1288_Y2664block_X2576_Y2664block_X3864_Y2664block_X5152_Y2664block_X6440_Y2664block_X7728_Y2664block_X9016_Y2664block_X10304_Y2664block_X11592_Y2664block_X12880_Y2664block_X14168_Y2664
block_X0_Y1332block_X1288_Y1332block_X2576_Y1332block_X3864_Y1332block_X5152_Y1332block_X6440_Y1332block_X7728_Y1332block_X9016_Y1332block_X10304_Y1332block_X11592_Y1332block_X12880_Y1332block_X14168_Y1332
block_X0_Y0block_X1288_Y0block_X2576_Y0block_X3864_Y0block_X5152_Y0block_X6440_Y0block_X7728_Y0block_X9016_Y0block_X10304_Y0block_X11592_Y0block_X12880_Y0block_X14168_Y0
CountPercentage
Total Addressable Wells148,603,956
‣ Wells with ISPs 130,032,373 88%
‣ Live ISPs129,447,477 100%
‣ Test Fragment ISPs899,009 <1%
‣ Library ISPs128,548,468 99%
CountPercentage
Library ISPs / Percent Enrichment128,548,468 100%
‣ Filtered: Polyclonal46,974,678 37%
‣ Filtered: Primer dimer572,183 <1%
‣ Filtered: Low quality7,993,745 6%
‣ Final Library Reads74,813,096 58%

Report Information

Analysis Info

Run Name R_2015_03_12_12_59_17_user_ION-156-ITlab_150312_ChIP_ITC44-45-48-49
Run Date 2015-03-12 11:01:14+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC49-wt2SET8
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_10-ITsamples_ITC44-wt1Input-bc2_ITC45-wt2Input-bc3_ITC48-wt1SET8-bc8_ITC49-wt2SET8-bc10
Barcode Set IonXpress
Analysis Name Auto_user_ION-156-ITlab_150312_ChIP_ITC44-45-48-49_221
Analysis Date 2015-03-12
runID BRUU9

Software Version

Torrent_Suite4.4
hostFW7DQV1
ion-analysis4.4.6-1
ion-chefupdates4.4.3
ion-dbreports4.4.22-1
ion-gpu4.4.1-1
ion-pipeline4.4.9-1
ion-plugins4.4.12-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1
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