Using tophat2 installation at /data/results/tools/align/tophat-2.0.12.Linux_x86_64/tophat2 TopHat v2.0.12 Using bowtie installation at /data/results/tools/align/bowtie2-2.2.3/bowtie2 /data/results/tools/align/bowtie2-2.2.3/bowtie2-align-s version 2.2.3 64-bit Built on do-dmxp-mac.win.ad.jhu.edu Thu May 29 17:14:09 EDT 2014 Compiler: gcc version 4.1.2 20080704 (Red Hat 4.1.2-54) Options: -O3 -m64 -msse2 -funroll-loops -g3 -DPOPCNT_CAPABILITY Sizeof {int, long, long long, void*, size_t, off_t}: {4, 8, 8, 8, 8, 8} [bam_header_read] EOF marker is absent. The input is probably truncated. [samopen] SAM header is present: 22 sequences. 12389975 reads; of these: 12389975 (100.00%) were unpaired; of these: 874113 (7.06%) aligned 0 times 3675543 (29.67%) aligned exactly 1 time 7840319 (63.28%) aligned >1 times 92.94% overall alignment rate [bam_sort_core] merging from 9 files... [bam_sort_core] merging from 24 files... [sam_header_read2] 22 sequences loaded. 26087787 uniq. mapped reads [bam_header_read] EOF marker is absent. The input is probably truncated. [samopen] SAM header is present: 22 sequences. 14843900 reads; of these: 14843900 (100.00%) were unpaired; of these: 967000 (6.51%) aligned 0 times 3253641 (21.92%) aligned exactly 1 time 10623259 (71.57%) aligned >1 times 93.49% overall alignment rate [bam_sort_core] merging from 10 files... [bam_sort_core] merging from 24 files... [sam_header_read2] 22 sequences loaded. 24573182 uniq. mapped reads