Using tophat2 installation at /data/results/tools/align/tophat-2.0.10.Linux_x86_64/tophat2 TopHat v2.0.10 Using bowtie installation at /data/results/tools/align/bowtie2-2.1.0/bowtie2 /data/results/tools/align/bowtie2-2.1.0/bowtie2-align version 2.1.0 64-bit Built on do-dmxp-mac.win.ad.jhu.edu Tue Feb 26 13:34:02 EST 2013 Compiler: gcc version 4.1.2 20080704 (Red Hat 4.1.2-54) Options: -O3 -m64 -msse2 -funroll-loops -g3 Sizeof {int, long, long long, void*, size_t, off_t}: {4, 8, 8, 8, 8, 8} [bam_header_read] EOF marker is absent. The input is probably truncated. [samopen] SAM header is present: 22 sequences. 22471256 reads; of these: 22471256 (100.00%) were unpaired; of these: 3875911 (17.25%) aligned 0 times 10025960 (44.62%) aligned exactly 1 time 8569385 (38.13%) aligned >1 times 82.75% overall alignment rate [bam_sort_core] merging from 14 files... [bam_sort_core] merging from 16 files... [sam_header_read2] 22 sequences loaded. 18499841 uniq. mapped reads [bam_header_read] EOF marker is absent. The input is probably truncated. [samopen] SAM header is present: 22 sequences. 21097935 reads; of these: 21097935 (100.00%) were unpaired; of these: 1226727 (5.81%) aligned 0 times 11988109 (56.82%) aligned exactly 1 time 7883099 (37.36%) aligned >1 times 94.19% overall alignment rate [bam_sort_core] merging from 14 files... [bam_sort_core] merging from 16 files... [sam_header_read2] 22 sequences loaded. 19739614 uniq. mapped reads [bam_header_read] EOF marker is absent. The input is probably truncated. [samopen] SAM header is present: 22 sequences. 16956026 reads; of these: 16956026 (100.00%) were unpaired; of these: 1112853 (6.56%) aligned 0 times 8547535 (50.41%) aligned exactly 1 time 7295638 (43.03%) aligned >1 times 93.44% overall alignment rate [bam_sort_core] merging from 12 files... [bam_sort_core] merging from 16 files... [sam_header_read2] 22 sequences loaded. 15750260 uniq. mapped reads [bam_header_read] EOF marker is absent. The input is probably truncated. [samopen] SAM header is present: 22 sequences. 15020967 reads; of these: 15020967 (100.00%) were unpaired; of these: 888841 (5.92%) aligned 0 times 8884199 (59.15%) aligned exactly 1 time 5247927 (34.94%) aligned >1 times 94.08% overall alignment rate [bam_sort_core] merging from 11 files... [bam_sort_core] merging from 16 files... [sam_header_read2] 22 sequences loaded. 14060711 uniq. mapped reads