Report for Auto_user_ION-165-IRLab_KR-MuSeek_150331_ATACseq_IRMu8-9_230

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]3,686.30
‣ Number of Q20 Bases [Mbp]2,782.87
Total Number of Reads40,734,782
Mean Length [bp]90
Longest Read [bp]384

Reference Genome Information

Genome NameHomo sapiens
Genome Size3095693981
Genome Versionhg19
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]2,559.702,090.941,798.16
Mean Length [bp]857668
Longest Alignment [bp]230220203
Mean Coverage Depth0.83×0.68×0.58×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C52%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,551,315
Avg Q17 read length48
50AQ17806,296

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 129,469,778 87%
Live ISPs129,311,250 100%
Test Fragment ISPs1,771,624 1%
Library ISPs127,539,626 99%
CountPercentage
Library ISPs / Percent Enrichment127,539,626 100%
Filtered: Polyclonal61,665,488 48%
Filtered: Primer dimer1,295 <1%
Filtered: Low quality27,106,142 21%
Final Library Reads40,734,782 32%

Report Information

Analysis Info

Run Name R_2015_03_31_11_57_46_user_ION-165-IRLab_KR-MuSeek_150331_ATACseq_IRMu8-9
Run Date 2015-03-31 09:00:09+00:00
Run Flows 520
Project IRLab_ATACseq
Sample IRMu9-R2
Library N/A
Reference hg19
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes IRlam_KRMuSeeksamples_IRMu8-Rep1-bc6_IRMu9-Rep2-bc10
Barcode Set MuSeek Barcode set 1
Analysis Name Auto_user_ION-165-IRLab_KR-MuSeek_150331_ATACseq_IRMu8-9_230
Analysis Date 2015-03-31
runID YFQV3

Software Version

Torrent_Suite4.4.2
hostFW7DQV1
ion-analysis4.4.8-1
ion-chefupdates4.4.5
ion-dbreports4.4.29-1
ion-gpu4.4.1-1
ion-pipeline4.4.11-1
ion-plugins4.4.14-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1