Report for Auto_user_ION-153-IRLab_KR-MuSeek_150303_ATACseq_IRMu5-6_218

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]7,159.19
‣ Number of Q20 Bases [Mbp]5,680.34
Total Number of Reads63,383,249
Mean Length [bp]112
Longest Read [bp]384

Reference Genome Information

Genome NameHomo sapiens
Genome Size3095693981
Genome Versionhg19
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]4,942.894,158.003,531.54
Mean Length [bp]968777
Longest Alignment [bp]217196192
Mean Coverage Depth1.60×1.34×1.14×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C83%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,950,779
Avg Q17 read length76
50AQ171,611,100

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 129,090,586 87%
Live ISPs128,843,125 100%
Test Fragment ISPs2,221,790 2%
Library ISPs126,621,335 98%
CountPercentage
Library ISPs / Percent Enrichment126,621,335 100%
Filtered: Polyclonal45,620,678 36%
Filtered: Primer dimer9,562 <1%
Filtered: Low quality19,161,305 15%
Final Library Reads63,383,249 50%

Report Information

Analysis Info

Run Name R_2015_03_03_12_03_59_user_ION-153-IRLab_KR-MuSeek_150303_ATACseq_IRMu5-6
Run Date 2015-03-03 10:05:56+00:00
Run Flows 520
Project IRLab_ATACseq
Sample IRMu6-gDNA
Library N/A
Reference hg19
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes IRLab_KR-2MuSeekSamples_IRMu6-gDNA-bc5_IRMu7-Nuclei-bc7
Barcode Set MuSeek Barcode set 1
Analysis Name Auto_user_ION-153-IRLab_KR-MuSeek_150303_ATACseq_IRMu5-6_218
Analysis Date 2015-03-03
runID UFL3R

Software Version

Torrent_Suite4.4
hostFW7DQV1
ion-analysis4.4.6-1
ion-chefupdates4.4.3
ion-dbreports4.4.22-1
ion-gpu4.4.1-1
ion-pipeline4.4.9-1
ion-plugins4.4.12-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1