Report for Auto_user_ION-138-IRLab_KR-MuSeek_150204_ATACseq_IRMu5_203

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]2,987.86
‣ Number of Q20 Bases [Mbp]2,285.93
Total Number of Reads33,399,011
Mean Length [bp]89
Longest Read [bp]358

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]2,001.921,685.771,547.89
Mean Length [bp]837570
Longest Alignment [bp]188174166
Mean Coverage Depth0.75×0.63×0.58×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C51%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num409,746
Avg Q17 read length46
50AQ17207,376

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 126,937,854 85%
Live ISPs126,783,425 100%
Test Fragment ISPs561,403 <1%
Library ISPs126,222,022 100%
CountPercentage
Library ISPs / Percent Enrichment126,222,022 100%
Filtered: Polyclonal66,373,440 53%
Filtered: Primer dimer5,330 <1%
Filtered: Low quality26,463,679 21%
Final Library Reads33,399,011 26%

Report Information

Analysis Info

Run Name R_2015_02_04_15_42_26_user_ION-138-IRLab_KR-MuSeek_150204_ATACseq_IRMu5
Run Date 2015-02-04 13:44:10+00:00
Run Flows 520
Project IRLab_ATACseq
Sample IRMu5-Nuclei
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes IRLab_KR-1MuSeekSample_IRMu5-Nuclei-bc2
Barcode Set MuSeek Barcode set 1
Analysis Name Auto_user_ION-138-IRLab_KR-MuSeek_150204_ATACseq_IRMu5_203
Analysis Date 2015-02-04
runID W5GVU

Software Version

Torrent_Suite4.2.1
Datacollect 2929
Graphics 34
LiveView 1835
OIA 4201
OS 21
hostFW7DQV1
ion-analysis4.2.18-1
ion-chefupdates4.2.0
ion-dbreports4.2.22-1
ion-gpu4.2.2-1
ion-pipeline4.2.12-1
ion-plugins4.2.28-1
ion-protonupdates4.2.2
ion-torrentr4.2.1-1