Report for Auto_user_ION-183-GPLab_150508_MCRNAseq_GPR4-7_262

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]4,258.85
‣ Number of Q20 Bases [Mbp]3,585.11
Total Number of Reads46,602,581
Mean Length [bp]91
Longest Read [bp]390

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]3,016.982,619.402,415.18
Mean Length [bp]807469
Longest Alignment [bp]273264248
Mean Coverage Depth1.14×0.99×0.91×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C78%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num6,593,269
Avg Q17 read length72
50AQ175,114,982

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 104,335,126 70%
Live ISPs103,131,134 99%
Test Fragment ISPs7,039,197 7%
Library ISPs96,091,937 93%
CountPercentage
Library ISPs / Percent Enrichment96,091,937 99%
Filtered: Polyclonal29,427,670 31%
Filtered: Primer dimer798,842 <1%
Filtered: Low quality19,292,010 20%
Final Library Reads46,602,581 48%

Report Information

Analysis Info

Run Name R_2015_05_08_14_07_24_user_ION-183-GPLab_150508_MCRNAseq_GPR4-7
Run Date 2015-05-08 11:09:39+00:00
Run Flows 520
Project GPlab_RNAseq
Sample GPR7-784
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes GPlab_MC-8RNAseq_GPR4-TgArs-787B-bc7_GPR7-CtrlArs-784B-bc5
Barcode Set IonXpressRNA
Analysis Name Auto_user_ION-183-GPLab_150508_MCRNAseq_GPR4-7_262
Analysis Date 2015-05-08
runID RIOB0

Software Version

Torrent_Suite4.4.3
hostFW7DQV1
ion-analysis4.4.11-1
ion-chefupdates4.4.5
ion-dbreports4.4.38-1
ion-gpu4.4.1-1
ion-pipeline4.4.15-1
ion-plugins4.4.23-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1