Report for Auto_user_ION-179-PGLab_150506_MCRNAseq_GPR2-5_249

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]6,811.21
‣ Number of Q20 Bases [Mbp]5,749.26
Total Number of Reads69,347,544
Mean Length [bp]98
Longest Read [bp]399

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]4,523.593,937.203,587.75
Mean Length [bp]827671
Longest Alignment [bp]277271257
Mean Coverage Depth1.70×1.48×1.35×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C47%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num2,598,816
Avg Q17 read length43
50AQ171,217,253

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

block_X0_Y9324block_X1288_Y9324block_X2576_Y9324block_X3864_Y9324block_X5152_Y9324block_X6440_Y9324block_X7728_Y9324block_X9016_Y9324block_X10304_Y9324block_X11592_Y9324block_X12880_Y9324block_X14168_Y9324
block_X0_Y7992block_X1288_Y7992block_X2576_Y7992block_X3864_Y7992block_X5152_Y7992block_X6440_Y7992block_X7728_Y7992block_X9016_Y7992block_X10304_Y7992block_X11592_Y7992block_X12880_Y7992block_X14168_Y7992
block_X0_Y6660block_X1288_Y6660block_X2576_Y6660block_X3864_Y6660block_X5152_Y6660block_X6440_Y6660block_X7728_Y6660block_X9016_Y6660block_X10304_Y6660block_X11592_Y6660block_X12880_Y6660block_X14168_Y6660
block_X0_Y5328block_X1288_Y5328block_X2576_Y5328block_X3864_Y5328block_X5152_Y5328block_X6440_Y5328block_X7728_Y5328block_X9016_Y5328block_X10304_Y5328block_X11592_Y5328block_X12880_Y5328block_X14168_Y5328
block_X0_Y3996block_X1288_Y3996block_X2576_Y3996block_X3864_Y3996block_X5152_Y3996block_X6440_Y3996block_X7728_Y3996block_X9016_Y3996block_X10304_Y3996block_X11592_Y3996block_X12880_Y3996block_X14168_Y3996
block_X0_Y2664block_X1288_Y2664block_X2576_Y2664block_X3864_Y2664block_X5152_Y2664block_X6440_Y2664block_X7728_Y2664block_X9016_Y2664block_X10304_Y2664block_X11592_Y2664block_X12880_Y2664block_X14168_Y2664
block_X0_Y1332block_X1288_Y1332block_X2576_Y1332block_X3864_Y1332block_X5152_Y1332block_X6440_Y1332block_X7728_Y1332block_X9016_Y1332block_X10304_Y1332block_X11592_Y1332block_X12880_Y1332block_X14168_Y1332
block_X0_Y0block_X1288_Y0block_X2576_Y0block_X3864_Y0block_X5152_Y0block_X6440_Y0block_X7728_Y0block_X9016_Y0block_X10304_Y0block_X11592_Y0block_X12880_Y0block_X14168_Y0
CountPercentage
Total Addressable Wells148,603,956
‣ Wells with ISPs 119,352,433 80%
‣ Live ISPs119,052,010 100%
‣ Test Fragment ISPs3,184,421 3%
‣ Library ISPs115,867,589 97%
CountPercentage
Library ISPs / Percent Enrichment115,867,589 100%
‣ Filtered: Polyclonal34,093,114 29%
‣ Filtered: Primer dimer623,205 <1%
‣ Filtered: Low quality11,812,606 10%
‣ Final Library Reads69,347,544 60%

Report Information

Analysis Info

Run Name R_2015_05_06_14_42_12_user_ION-179-PGLab_150506_MCRNAseq_GPR2-5
Run Date 2015-05-06 11:44:52+00:00
Run Flows 520
Project GPlab_RNAseq
Sample GPR5-788
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes GPlab_MC-8RNAseq_GPR2-TgSal-786-bc2_GPR5-DtrlSal-788-bc5
Barcode Set IonXpressRNA
Analysis Name Auto_user_ION-179-PGLab_150506_MCRNAseq_GPR2-5_249
Analysis Date 2015-05-06
runID 5OUY5

Software Version

Torrent_Suite4.4.3
hostFW7DQV1
ion-analysis4.4.11-1
ion-chefupdates4.4.5
ion-dbreports4.4.38-1
ion-gpu4.4.1-1
ion-pipeline4.4.15-1
ion-plugins4.4.23-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1
Request Support  |  Help/Documentation  |  Terms of Use
Copyright © 2012 Life Technologies Corporation
This product should be used for research use only