cd /data/images/proton2/run416/wwwGKlab
samtools merge GK3R215_wt1.bam  GK3R215_wt1.bam1  GK3R215_wt1.bam2  GK3R215_wt1.bam3
samtools merge GK3R216_wt2.bam  GK3R216_wt2.bam1  GK3R216_wt2.bam2  GK3R216_wt2.bam3
samtools merge GK3R217_wt3.bam  GK3R217_wt3.bam1  GK3R217_wt3.bam2  GK3R217_wt3.bam3
samtools merge GK3R220_DARE4.bam  GK3R220_DARE4.bam1  GK3R220_DARE4.bam2  GK3R220_DARE4.bam3
samtools merge GK3R226_DARETie3.bam  GK3R226_DARETie3.bam1  GK3R226_DARETie3.bam2  GK3R226_DARETie3.bam3

]0;/data/images/proton2/run411/wwwreczko@max:/data/images/proton2/run411/www$ awk -f /data/images/proton/make-metaseqr-targets1.awk ../samples.txt > targets.txt

the.contrasts.1 <- c(
"wt_vs_DARE",
"wt_vs_DAREp75dd",
"wt_vs_DARETie",
"DARE_vs_DAREp75dd",
"DARE_vs_DARETie",
"DAREp75dd_vs_DARETie")
the.path="/data/images/proton2/run416/wwwGKlab"

metaseqr(
    sample.list=file.path(the.path,"targets.txt"),
    contrast=the.contrasts.1,
    annotation="download",
    org="mm10",
    count.type="utr",
    normalization="deseq",
    statistics="deseq",
    fig.format=c("png","pdf"),
    export.where=file.path(the.path,"metaseqr_quantseq_MSakkou_run411_416"),
    restrict.cores=0.5,
    qc.plots=c(
        "mds","biodetection","countsbio","saturation","readnoise","filtered",
        "correl","pairwise","boxplot","gcbias","lengthbias","meandiff",
        "meanvar","biodist","volcano","deheatmap"
    ),
    exon.filters=NULL,
    gene.filters=list(
       length=list(
            length=500
        ),
        avg.reads=list(
            average.per.bp=100,
            quantile=0.25
        ),
        expression=list(
            median=TRUE,
            mean=FALSE,
            quantile=NA,
            known=NA,
            custom=NA
        ),
        biotype=get.defaults("biotype.filter","mm10")
    ),
    pcut=0.05,
    export.what=c("annotation","p.value","adj.p.value","fold.change",
		"counts","flags"),
    export.scale=c("log2","rpgm"),
    export.values="normalized",
    export.counts.table=TRUE,
	report.top=0.05
)