Dear Fani, the analysis of your QUANTseq experiment has finished. The samples and the number of reads obtained are: sample n_reads GK3R188-Wt1 1841923 GK3R189-Wt2 5287804 GK3R190-C1 5022295 GK3R191-C2 5098218 GK3R192-C3 6231647 GK3R193-Tg197-1 6337949 GK3R194-Tg197-2 6923642 GK3R195-Tg197-3 7029048 GK3R196-Tg197-C1 6158257 GK3R197-Tg197-C2 1642910 GK3R198-Tg197-C3 4487739 At http://genomics-lab.fleming.gr/fleming/GKlab/run403/metaseqr_GKlab_run403/ you will find the metaseqR [1] differential gene expression analysis using the contrasts LiveInv_vs_FIXED, which is (note the used naming convention: in A_vs_B, A is always the reference). In the "Results" section, there are links to download the diff. expr. results under "DEG table", "Download the whole result list". Based on the MDS plot and the smaller number of reads, I'd recommend a reanalysis with GK3R197-Tg197-C2 omitted. With best regards, Martin [1] Moulos P and Hatzis P “Systematic integration of RNA-Seq statistical algorithms for accurate detection of differential gene expression patterns.” Nucl. Acids Res. (2014) doi: 10.1093/nar/gku1273 http://nar.oxfordjournals.org/content/early/2014/12/01/nar.gku1273.abstract.