Report for Auto_user_IONAS-340-GKlab_170327_GK3R87-98_439

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]6,321.18
‣ Number of Q20 Bases [Mbp]5,157.27
Total Number of Reads52,291,050
Mean Length [bp]120
Longest Read [bp]390

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]1,374.66755.80
Mean Length [bp]10882
Longest Alignment [bp]331307
Mean Coverage Depth0.50×0.28×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_195%
TF_C94%

Test Fragment - TF_1

Quality Metrics

TF NameTF_1
TF SeqGAATAATCCAGCCCGCCAGGCATGGAAGAGCGTCGTAAAGTATTGCAGGT TCAGGCGGCGGAAAGCGTGATTGACTACTGGCAAATAAAGTACGTTCCAC CTTTGACACCATTTTCCGTAGTGAACTGACGCTGCCAAACGCCGACCGCG
Num16,979
Avg Q17 read length141
50AQ1716,197

Graphs

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,804,586
Avg Q17 read length89
50AQ171,690,814

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 102,484,210 69%
Live ISPs101,725,534 99%
Test Fragment ISPs1,884,801 2%
Library ISPs99,840,733 98%
CountPercentage
Library ISPs / Percent Enrichment99,840,733 99%
Filtered: Polyclonal30,798,118 31%
Filtered: Primer dimer746,391 <1%
Filtered: Low quality16,584,051 17%
Final Library Reads52,803,095 53%

Report Information

Analysis Info

Run Name R_2017_03_28_18_54_03_user_IONAS-340-GKlab_170327_GK3R87-98
Run Date 2017-03-28 15:56:48+00:00
Run Flows 520
Project LexoGen_QuantSeq,GKLab_3RNAseq
Sample GK3R95-TI3
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes GKlab_12VKoliarakiRNAs_GK3R87-98
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-340-GKlab_170327_GK3R87-98_439
Analysis Date 2017-03-28
runID 7D2TC

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1