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Report for Auto_user_IONAS-339-GKlab_170327_GK3R99-110_440

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]8,598.56
‣ Number of Q20 Bases [Mbp]6,854.02
Total Number of Reads67,764,329
Mean Length [bp]126
Longest Read [bp]390

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]1,931.321,057.64
Mean Length [bp]10072
Longest Alignment [bp]326308
Mean Coverage Depth0.71×0.39×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C88%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num2,094,430
Avg Q17 read length83
50AQ171,847,415

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 114,698,075 77%
Live ISPs114,065,926 99%
Test Fragment ISPs2,132,438 2%
Library ISPs111,933,488 98%
CountPercentage
Library ISPs / Percent Enrichment111,933,488 99%
Filtered: Polyclonal31,302,474 28%
Filtered: Primer dimer198,681 <1%
Filtered: Low quality13,657,037 12%
Final Library Reads68,199,272 61%

Report Information

Analysis Info

Run Name R_2017_03_28_11_55_53_user_IONAS-339-GKlab_170327_GK3R99-110
Run Date 2017-03-28 08:57:38+00:00
Run Flows 520
Project LexoGen_QuantSeq,GKLab_3RNAseq
Sample GK3R106_TR2
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes GKlab_12DPapadopoulouRNAs_GK3R99-110
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-339-GKlab_170327_GK3R99-110_440
Analysis Date 2017-03-28
runID 4UOGV

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1