Report for Auto_user_IONAS-317-DrSpilian_GKlab_161122_BSC1r-4r_GK3R78-86_415

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]7,555.25
‣ Number of Q20 Bases [Mbp]5,785.25
Total Number of Reads67,243,421
Mean Length [bp]112
Longest Read [bp]390

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]3,273.632,494.28
Mean Length [bp]9579
Longest Alignment [bp]306291
Mean Coverage Depth1.20×0.92×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C67%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,714,460
Avg Q17 read length62
50AQ171,157,127

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 131,964,832 89%
Live ISPs131,592,197 100%
Test Fragment ISPs2,271,736 2%
Library ISPs129,320,461 98%
CountPercentage
Library ISPs / Percent Enrichment129,320,461 100%
Filtered: Polyclonal47,981,228 37%
Filtered: Primer dimer96,707 <1%
Filtered: Low quality13,399,492 10%
Final Library Reads67,877,678 52%

Report Information

Analysis Info

Run Name R_2016_11_22_11_28_55_user_IONAS-317-DrSpilian_GKlab_161122_BSC1r-4r_GK3R78-86
Run Date 2016-11-22 09:30:30+00:00
Run Flows 520
Project DrSpilianakis_ChIPseq,LexoGen_QuantSeq,GKLab_3RNAseq
Sample BSC4r-I
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes DrSpilian_RepeatSeq160524-afterFragmofSamples_GKlab-VK9-3RNAseq
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-317-DrSpilian_GKlab_161122_BSC1r-4r_GK3R78-86_415
Analysis Date 2016-11-22
runID JB9UG

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1