Report for Auto_user_IONAS-307-GKlab_MS_160928_GK3R55--77_405

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]8,398.35
‣ Number of Q20 Bases [Mbp]6,543.26
Total Number of Reads78,080,069
Mean Length [bp]107
Longest Read [bp]385

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]65.7345.51
Mean Length [bp]9176
Longest Alignment [bp]276240
Mean Coverage Depth0.02×0.02×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C72%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num900,350
Avg Q17 read length65
50AQ17651,423

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 133,836,450 90%
Live ISPs133,359,888 100%
Test Fragment ISPs1,303,422 <1%
Library ISPs132,056,466 99%
CountPercentage
Library ISPs / Percent Enrichment132,056,466 100%
Filtered: Polyclonal45,585,424 35%
Filtered: Primer dimer250,883 <1%
Filtered: Low quality8,381,156 6%
Final Library Reads78,532,816 59%

Report Information

Analysis Info

Run Name R_2016_09_28_11_13_05_user_IONAS-307-GKlab_MS_160928_GK3R55--77
Run Date 2016-09-28 08:14:43+00:00
Run Flows 520
Project LexoGen_QuantSeq,GKLab_3RNAseq
Sample GK3R75r-MS22
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes GK3R55--77_MSakkou_14samples_7resequenced_7newlibrary
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-307-GKlab_MS_160928_GK3R55--77_405
Analysis Date 2016-09-28
runID D13ZT

Software Version

Torrent_Suite5.0.4
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.3
ion-dbreports5.0.33-1
ion-gpu5.0.0-1
ion-pipeline5.0.16-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1