Report for Auto_user_IONAS-303-GKlab_MA_160909_GK3R39-53_401

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]8,514.00
‣ Number of Q20 Bases [Mbp]6,710.65
Total Number of Reads81,751,247
Mean Length [bp]104
Longest Read [bp]394

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]2,726.671,958.77
Mean Length [bp]8774
Longest Alignment [bp]284284
Mean Coverage Depth1.00×0.72×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C79%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num2,570,574
Avg Q17 read length71
50AQ172,034,576

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 133,776,465 90%
Live ISPs133,295,755 100%
Test Fragment ISPs3,055,122 2%
Library ISPs130,240,633 98%
CountPercentage
Library ISPs / Percent Enrichment130,240,633 100%
Filtered: Polyclonal40,110,211 31%
Filtered: Primer dimer88,912 <1%
Filtered: Low quality9,227,281 7%
Final Library Reads82,107,695 63%

Report Information

Analysis Info

Run Name R_2016_09_09_12_00_04_user_IONAS-303-GKlab_MA_160909_GK3R39-53
Run Date 2016-09-09 09:01:28+00:00
Run Flows 520
Project LexoGen_QuantSeq,GKLab_3RNAseq
Sample GK3R53-MA44
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes GK3R39-53_MArmaka_final15of44samples
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-303-GKlab_MA_160909_GK3R39-53_401
Analysis Date 2016-09-09
runID N1ZBX

Software Version

Torrent_Suite5.0.4
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.3
ion-dbreports5.0.33-1
ion-gpu5.0.0-1
ion-pipeline5.0.16-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1