Dear Pantelis,

(after serveral updates needed),
I've merged run 200+201  and 202+205.
and sampled all merged libs to:
IonXpressRNA_008	GKR27-III1h
having  45548448 reads.
The tracks are ready at:
http://genomics-lab.fleming.gr/cgi-bin/hgTracks?db=mm9&hubUrl=http://genomics-lab.fleming.gr/fleming/GKlab/run205/hub.txt
BW,
Martin


 Dear Pantelis,

these are the mapping stats for the 4 GKlab runs
run200/samples.txt
Barcode Name	Sample	Bases	>=Q20 Bases	Reads	Mean Read Length
No barcode	None	126828313	82496818	1482297	86 bp 
IonXpressRNA_007	GKR26-III0h	2265239933	1804203115	19027698	119 bp 
IonXpressRNA_008	GKR27-III1h	2124572081	1694701172	18652898	114 bp 
007.tophat-mm9a.log 19027698 reads 18485638 aligned 97.1512 pct_aligned
008.tophat-mm9a.log 18652898 reads 18060839 aligned 96.8259 pct_aligned

run201/samples.txt
Barcode Name	Sample	Bases	>=Q20 Bases	Reads	Mean Read Length
No barcode	None	143121476	94767881	1536651	93 bp 
IonXpressRNA_007	GKR26-III0h	3290677369	2574872290	27553241	119 bp 
IonXpressRNA_008	GKR27-III1h	3078286491	2406426246	26895550	114 bp 
007.tophat-mm9a.log 27553241 reads 26844009 aligned 97.426 pct_aligned
008.tophat-mm9a.log 26895550 reads 26115863 aligned 97.1011 pct_aligned

run202/samples.txt
Barcode Name	Sample	Bases	>=Q20 Bases	Reads	Mean Read Length
No barcode	None	150245735	97475964	1613115	93 bp 
IonXpressRNA_009	GKR28-III3h	2295914369	1741867501	18101311	127 bp 
IonXpressRNA_010	GKR29-III6h	2685210659	2101075510	22417500	120 bp 
009.tophat-mm9a.log 18101311 reads 17493492 aligned 96.6421 pct_aligned
010.tophat-mm9a.log 22417500 reads 21657265 aligned 96.6087 pct_aligned

run205/samples.txt
Barcode Name	Sample	Bases	>=Q20 Bases	Reads	Mean Read Length
No barcode	None	233000491	181251575	1717524	136 bp 
IonXpressRNA_009	GKR28-III3h	5135827759	4176381903	34256443	150 bp 
IonXpressRNA_010	GKR29-III6h	6739785371	5585693967	49391731	136 bp 
009.tophat-mm9a.log 34256443 reads 33771531 aligned 98.5845 pct_aligned
010.tophat-mm9a.log 49391731 reads 48428324 aligned 98.0495 pct_aligned

For the tracks,
I'll merge run 200+201  and 202+205.
and sample to:
IonXpressRNA_008	GKR27-III1h
having 18.7+26.9=45.6 Mreads
as the smallest lib.
ok?

BW
Martin