Report for Auto_user_ION-205-GKlab_VNRNAseq_150708_CHEF_GKR28-29_289

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]12,108.80
‣ Number of Q20 Bases [Mbp]9,943.48
Total Number of Reads85,367,041
Mean Length [bp]141
Longest Read [bp]389

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]7,075.555,986.554,932.83
Mean Length [bp]1089883
Longest Alignment [bp]326309296
Mean Coverage Depth2.67×2.25×1.86×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C70%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num599,013
Avg Q17 read length65
50AQ17417,326

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 132,717,466 89%
Live ISPs132,647,524 100%
Test Fragment ISPs690,480 <1%
Library ISPs131,957,044 99%
CountPercentage
Library ISPs / Percent Enrichment131,957,044 100%
Filtered: Polyclonal41,921,423 32%
Filtered: Primer dimer158,252 <1%
Filtered: Low quality4,555,006 3%
Final Library Reads85,367,041 65%

Report Information

Analysis Info

Run Name R_2015_07_10_12_30_50_user_ION-205-GKlab_VNRNAseq_150708_CHEF_GKR28-29_
Run Date 2015-07-10 09:32:58+00:00
Run Flows 520
Project GKLab_RNASeq
Sample GKR29-III6h
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes GKlab_VN-18RNAsamples_GKR28-III3h-bc9_GKR29-III6h-bc10
Barcode Set IonXpressRNA
Analysis Name Auto_user_ION-205-GKlab_VNRNAseq_150708_CHEF_GKR28-29_289
Analysis Date 2015-07-10
runID ALDG1

Software Version

Torrent_Suite4.4.3
hostFW7DQV1
ion-analysis4.4.11-1
ion-chefupdates4.4.5
ion-dbreports4.4.38-1
ion-gpu4.4.1-1
ion-pipeline4.4.15-1
ion-plugins4.4.23-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1