Dear Panagiotis as discussed, some RNAseq metaseqR analysis was treated as unstranded due to a small 'glitch' in metaseqR when the update for 3UTRseq was introduced. This had a very small effect on expression values. The affected reports have been regenerated at: http://genomics-lab.fleming.gr/fleming/GKlab/run339/metaseqr_quantseq_run339_with_UNK_stranded http://genomics-lab.fleming.gr/fleming/GKlab/run340/metaseqr_quantseq_run340_without_GIH2_stranded As you suggested, the previous results can be kept under the unstranded restriction. Best wishes, Martin