Using tophat2 installation at /data/results/tools/align/tophat-2.0.12.Linux_x86_64/tophat2 TopHat v2.0.12 Using bowtie installation at /data/results/tools/align/bowtie2-2.2.3/bowtie2 /data/results/tools/align/bowtie2-2.2.3/bowtie2-align-s version 2.2.3 64-bit Built on do-dmxp-mac.win.ad.jhu.edu Thu May 29 17:14:09 EDT 2014 Compiler: gcc version 4.1.2 20080704 (Red Hat 4.1.2-54) Options: -O3 -m64 -msse2 -funroll-loops -g3 -DPOPCNT_CAPABILITY Sizeof {int, long, long long, void*, size_t, off_t}: {4, 8, 8, 8, 8, 8} [bam_header_read] EOF marker is absent. The input is probably truncated. [samopen] SAM header is present: 22 sequences. 18539178 reads; of these: 18539178 (100.00%) were unpaired; of these: 1715855 (9.26%) aligned 0 times 5914149 (31.90%) aligned exactly 1 time 10909174 (58.84%) aligned >1 times 90.74% overall alignment rate [bam_sort_core] merging from 12 files... [bam_sort_core] merging from 32 files... [sam_header_read2] 22 sequences loaded. 40965050 uniq. mapped reads [bam_header_read] EOF marker is absent. The input is probably truncated. [samopen] SAM header is present: 22 sequences. 20570121 reads; of these: 20570121 (100.00%) were unpaired; of these: 1665602 (8.10%) aligned 0 times 5931301 (28.83%) aligned exactly 1 time 12973218 (63.07%) aligned >1 times 91.90% overall alignment rate [bam_sort_core] merging from 14 files... [bam_sort_core] merging from 32 files... [sam_header_read2] 22 sequences loaded. 42063858 uniq. mapped reads