Dear Vasso, the analysis of your 3RNAseq experiment has finished. The samples and the number of reads obtained are: VK3R1_ColVI-unst1 5495249 VK3R2_ColVI-unst2 7483179 VK3R3_ColVI-unst3 4794461 VK3R4_ColVI-APCst1 5593562 VK3R5_ColVI-APCst2 6129352 VK3R6_ColVI-APCst3 5660793 At http://genomics-lab.fleming.gr/cgi-bin/hgTracks?db=mm10&hubUrl=http://genomics-lab.fleming.gr/fleming/GKlab/Kolliaraki/run412/hub.txt you will find tracks for the samples, normalized to the smallest library, VK3R3_ColVI-unst3 with 4794461 reads. At http://genomics-lab.fleming.gr/fleming/GKlab/Kolliaraki/run412/metaseqr_quantseq_run412/ you will find the metaseqR [1] differential gene expression analysis using the contrast unst_vs_APCst (note the used naming convention: in A_vs_B, A is always the reference). In the "Results" section, there are links to download the diff. expr. results under "DEG table", "Download the whole result list". With best regards, Martin Reczko [1] Moulos P and Hatzis P “Systematic integration of RNA-Seq statistical algorithms for accurate detection of differential gene expression patterns.” Nucl. Acids Res. (2014) doi: 10.1093/nar/gku1273 http://nar.oxfordjournals.org/content/early/2014/12/01/nar.gku1273.abstract.