Dear Dr. Skoulakis, the analysis of your RNAseq experiment has finished. The samples and the number of reads obtained are: ES3R1_C 9826457 ES3R2_M 7749211 ES3R3_C2 6479496 ES3R4_2 6855847 At http://genomics-lab.fleming.gr/cgi-bin/hgTracks?db=dm3&hubUrl=http://genomics-lab.fleming.gr/fleming/ESlab/run430/hub.txt (within Fleming: http://filia/cgi-bin/hgTracks?db=dm3&hubUrl=http://genomics-lab.fleming.gr/fleming/ESlab/run430/hub.txt ) you will find tracks for all samples integrated in our UCSC genome browser mirror. These tracks are sampled to smallest library (ES3R3_C2 with 6479496 reads). At http://genomics-lab.fleming.gr/fleming/ESlab/run430/metaseqr_ESlab_run430 (within Fleming: http://filia/fleming/ESlab/run430/metaseqr_ESlab_run430 ) you will find the metaseqR [1] differential gene expression analysis using the contrast LiveInv_vs_FIXED, which is (note the used naming convention: in A_vs_B, A is always the reference). In the "Results" section, there are links to download the diff. expr. results under "DEG table", "Download the whole result list". With best regards, Martin Reczko [1] Moulos P and Hatzis P “Systematic integration of RNA-Seq statistical algorithms for accurate detection of differential gene expression patterns.” Nucl. Acids Res. (2014) doi: 10.1093/nar/gku1273 http://nar.oxfordjournals.org/content/early/2014/12/01/nar.gku1273.abstract. Dear Pantelis, for the ESlab, the samples are: ES3R1_C ES3R2_M ES3R3_C2 ES3R4_2 What is the contrast? BW Martin #@ C is control, non_C is mutant