MR12072017 reczko@estia:/mnt/raid/data/results/fleming/dk$ samtools sort sortedKo01pos.bam.bam sortedKo01pos.sorted.bam samtools sort sortedKo02pos.bam.bam sortedKo02pos.sorted.bam samtools flagstat sortedKo01pos.sorted.bam 87478404 in total 0 QC failure 0 duplicates 87478404 mapped (100.00%) 87478404 paired in sequencing 43739202 read1 43739202 read2 83948534 properly paired (95.96%) 87478404 with itself and mate mapped 0 singletons (0.00%) 0 with mate mapped to a different chr 0 with mate mapped to a different chr (mapQ>=5) samtools flagstat sortedKo01pos.bam.bam 87478404 in total 0 QC failure 0 duplicates 87478404 mapped (100.00%) 87478404 paired in sequencing 43739202 read1 43739202 read2 83948534 properly paired (95.96%) 87478404 with itself and mate mapped 0 singletons (0.00%) 0 with mate mapped to a different chr 0 with mate mapped to a different chr (mapQ>=5) for i in *bam do samtools index $i echo $i done Dear Dimitris and Yiota, I've copied the files Orsalia specified and prepared them for visualization in IGV (sorted the alignments and added an .bai index file). If you load the mm9 genome in IGV, you can add each bam file using File -> Load from URL and the following links: http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedKO21pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedKO22pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedKOIFN1pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedKOIFN2pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedKo01pos.sorted.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedKo02pos.sorted.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedKo1IL4pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedKo2IL4pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedKo61pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedKo62pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedwt01IL4pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedwt01pos.bam.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedwt02IL4pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedwt02pos.bam.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedwt21pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedwt22pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedwt61pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedwt62pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedwtIFN1pos.bam http://genomics-lab.fleming.gr/fleming/DKlab/orsalia/sortedwtIFN2pos.bam BW, Martin