FastQCFastQC Report
Fri 3 Nov 2017
KO_0h_replicate_I.bam

Summary

[OK]Basic Statistics

MeasureValue
FilenameKO_0h_replicate_I.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5998807
Sequences flagged as poor quality0
Sequence length25-75
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGTC22350.0217.94669
GCTGTCT13150.0196.0280269
CGGCTGT17450.0193.1110568
TTCTCAA59900.0181.6353969
CGAACGG500.0172.3005265
CACGGCT25700.0166.90166
ACGGCTG17100.0164.7263567
ACGGCCG64050.0163.0225768
CGGCCGG70400.0157.5751869
CCACGGC26200.0149.612165
TCGTGAT10250.0147.6600567
CCCACGG25950.0147.0784364
CACGGCC53300.0146.4505867
ACCCACG26850.0140.9253263
CGGCGCG1150.0136.0187264
GCACGGC50450.0134.7966566
CGTGATG11800.0134.0728868
CACGAGA63550.0132.9734367
CGCACGG70950.0131.2896165
CGACGAG1300.0129.3923266
Produced by FastQC (version 0.11.5)