FastQCFastQC Report
Fri 10 Nov 2017
0hrep1b.bam

Summary

[OK]Basic Statistics

MeasureValue
Filename0hrep1b.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26909731
Sequences flagged as poor quality0
Sequence length18-74
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACC204200.078.499651
AAATCGT294800.076.8027246
AATCGTA258750.076.6035447
CAATAAA540050.076.5428442
AATTGTA125100.075.509647
GAGACAA586700.075.1354838
ACAATAA572950.074.2733641
GACAATA513350.074.1867340
AGACAAT606600.073.960839
AAATTGT157950.073.72491546
GAGCCAA183150.072.986138
TAAATCG295500.072.79584545
TAAATGG107050.072.32271645
CTTCCTA88600.071.1709764
CGGAGAC399850.070.21442436
GGAGACA547500.069.35754437
AGGCAAT77200.069.189639
AGCCAAT100550.068.9476439
TAAATTG156400.068.110545
GTAGCCC86600.067.9545551
Produced by FastQC (version 0.11.5)