FastQCFastQC Report
Fri 10 Nov 2017
0hrep1b2.bam

Summary

[OK]Basic Statistics

MeasureValue
Filename0hrep1b2.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28066949
Sequences flagged as poor quality0
Sequence length13-74
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGACC202600.079.2190151
AAATCGT296000.077.85895546
AATCGTA256800.077.6343747
CAATAAA532600.077.0299542
GAGACAA583200.075.9818838
AGACAAT600950.074.9873939
GACAATA504350.074.9067640
ACAATAA561450.074.8408641
AATTGTA127900.074.6769547
TAAATCG290200.074.225645
GAGCCAA179350.073.3929538
CTTCCTA88050.072.6024464
AAATTGT155550.072.5030646
TAAATGG100000.072.39989545
AGGCAAT78400.071.2469539
CGGAGAC395250.070.6779836
AGTTAGG105150.070.0318265
GGAGACA551200.069.9171437
CAGTTAG103250.068.940964
TAAATTG148250.068.4219345
Produced by FastQC (version 0.11.5)