Cufflinks report, contrast M14_vs_M14mut

The samples have the following names, conditions, replicate number and number of reads:

sample_name      condition_replicateID    n_reads
_________________________________________________
SC3R1-1M14       M14_0                    6710396
SC3R2-3M14       M14_1                    4694087
SC3R3-6M14mut    M14mut_0                 4350420
SC3R4-8M14mut    M14mut_1                 3935188
SC3R5-9M14mut    M14mut_2                 4735942





Normalized UCSC genome browser tracks

Tracks normalized to the smallest library
SC3R6-1F16 with 3658897 reads are at:

http://genomics-lab.fleming.gr/cgi-bin/hgTracks?db=mm10&hubUrl=http://genomics-lab.fleming.gr/fleming/Cereghini/run299/hub.txt


Differentially expressed genes (DEGs)

A speadsheet with differential expression information for the contrast M14_vs_M14mut for all known gense and novel genes identified by cufflinks are at:

http://genomics-lab.fleming.gr/fleming/Cereghini/run299/cuffdiff_M14_vs_M14mut/gene_exp.diff.xlsx


The number of known and novel DEG up to q-value 0.05 is 1041
The number of known DEG up to q-value 0.05 is 66
The number of known and novel DEG up to q-value 0.01 is 691
The number of known DEG up to q-value 0.01 is 71



Differentially expressed isoforms

A speadsheet with differential expression information for the contrast M14_vs_M14mut for all known isoforms and novel isoforms found by cufflinks including biotype information is at:

http://genomics-lab.fleming.gr/fleming/Cereghini/run299/cuffdiff_M14_vs_M14mut/isoform_exp.diff.xlsx




Multi-Dimensional Scaling plot

Multi-Dimensional Scaling (MDS) plots comprise a means of visualizing the level of similarity of individual cases of a dataset. MDS uses absolute distance metrics such as the classical Euclidean distance as a similarity measure. MDS serves as a quality control and it can be interpreted as follows: when the distance among samples of the same biological condition in the MDS space is small, this is an indication of high correlation and reproducibility among them. When this distance is larger, this constitutes an indication of low correlation and reproducibility among samples. It can help to identify mislabled samples or to exclude poor samples from further analysis.



MDS plot based on gene expression




Volcano plot

A volcano plot is a scatterplot to give an overview of interesting genes. The log2 fold change is plotted on the x-axis and the negative log10 p-value is plotted on the y-axis. A volcano plot combines the results of a statistical test (aka, p-values) with the magnitude of the change enabling quick visual identification of those genes that display large-magnitude changes that are also statistically significant.




Additional files

All files of the cufflinks/cuffdiff analysis can be found at:

http://genomics-lab.fleming.gr/fleming/Cereghini/run299/cuffdiff_M14_vs_M14mut

The format of all files is explained at http://cole-trapnell-lab.github.io/cufflinks/cuffdiff/index.html#cuffdiff-output-files.
References and more information can be found at http://cole-trapnell-lab.github.io/cufflinks/papers.




This report was made on Fri Jul 22 16:09:46 2016 with the Cufflinks report generator by Martin Reczko.