This is cutadapt 1.9.1 with Python 3.6.1 Command line parameters: -a AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -o 131218_SND393_A_L006_GZS-17_R1.trm.fq.gz 131218_SND393_A_L006_GZS-17_R1.fastq.gz Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4954.01 s (90 us/read; 0.67 M reads/minute). === Summary === Total reads processed: 54,969,680 Reads with adapters: 3,973,117 (7.2%) Reads written (passing filters): 54,969,680 (100.0%) Total basepairs processed: 5,496,968,000 bp Total written (filtered): 5,453,752,514 bp (99.2%) === Adapter 1 === Sequence: AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC; Type: regular 3'; Length: 34; Trimmed: 3973117 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-34 bp: 3 Bases preceding removed adapters: A: 15.0% C: 32.6% G: 34.3% T: 18.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1227455 858901.2 0 1227455 4 381084 214725.3 0 381084 5 213166 53681.3 0 213166 6 159859 13420.3 0 159859 7 147078 3355.1 0 147078 8 136824 838.8 0 136824 9 129617 209.7 0 128478 1139 10 121986 52.4 1 118549 3437 11 113439 13.1 1 110792 2647 12 106431 3.3 1 103913 2518 13 99337 0.8 1 97030 2307 14 93059 0.2 1 90819 2240 15 86810 0.1 1 84497 2313 16 79791 0.0 1 77536 2255 17 73534 0.0 1 71189 2345 18 68718 0.0 1 66454 2203 61 19 61708 0.0 1 59434 2151 123 20 56985 0.0 2 54449 2255 281 21 52913 0.0 2 50562 2115 236 22 48644 0.0 2 46437 1943 264 23 44863 0.0 2 42850 1801 212 24 42496 0.0 2 40554 1710 232 25 38940 0.0 2 36919 1794 227 26 36027 0.0 2 34133 1690 204 27 33204 0.0 2 31411 1575 212 6 28 29952 0.0 2 28309 1417 166 60 29 26725 0.0 2 25166 1371 149 39 30 24438 0.0 3 22937 1248 133 120 31 21994 0.0 3 20436 1309 171 78 32 20207 0.0 3 18832 1141 146 88 33 18830 0.0 3 17557 1098 118 57 34 17488 0.0 3 16289 1061 91 47 35 15549 0.0 3 14427 976 107 39 36 14394 0.0 3 13380 864 114 36 37 12699 0.0 3 11831 743 71 54 38 11640 0.0 3 10831 696 74 39 39 10443 0.0 3 9744 599 61 39 40 9435 0.0 3 8773 565 67 30 41 8333 0.0 3 7758 489 55 31 42 7642 0.0 3 7100 480 44 18 43 6824 0.0 3 6309 433 50 32 44 6223 0.0 3 5804 359 34 26 45 5880 0.0 3 5456 354 43 27 46 5254 0.0 3 4907 290 33 24 47 4829 0.0 3 4522 263 32 12 48 4350 0.0 3 4060 246 26 18 49 3953 0.0 3 3685 228 26 14 50 3506 0.0 3 3262 210 23 11 51 3182 0.0 3 2969 183 17 13 52 2739 0.0 3 2564 151 14 10 53 2395 0.0 3 2237 128 22 8 54 2169 0.0 3 2022 127 15 5 55 1925 0.0 3 1779 125 14 7 56 1712 0.0 3 1607 90 10 5 57 1564 0.0 3 1460 90 9 5 58 1485 0.0 3 1376 97 8 4 59 1242 0.0 3 1150 75 11 6 60 1086 0.0 3 1025 55 4 2 61 903 0.0 3 838 58 3 4 62 722 0.0 3 679 38 1 4 63 625 0.0 3 590 32 2 1 64 570 0.0 3 528 38 2 2 65 488 0.0 3 464 20 3 1 66 412 0.0 3 381 27 1 3 67 352 0.0 3 323 24 2 3 68 295 0.0 3 278 12 2 3 69 197 0.0 3 180 16 0 1 70 162 0.0 3 153 8 0 1 71 147 0.0 3 138 7 1 1 72 110 0.0 3 100 9 0 1 73 88 0.0 3 82 5 0 1 74 70 0.0 3 65 5 75 76 0.0 3 71 3 1 1 76 79 0.0 3 72 5 1 1 77 105 0.0 3 100 4 0 1 78 82 0.0 3 77 4 0 1 79 96 0.0 3 84 10 1 1 80 112 0.0 3 108 4 81 114 0.0 3 105 7 2 82 138 0.0 3 131 6 1 83 187 0.0 3 170 16 0 1 84 190 0.0 3 183 6 0 1 85 203 0.0 3 191 11 0 1 86 190 0.0 3 172 15 1 2 87 175 0.0 3 166 7 2 88 192 0.0 3 180 10 1 1 89 169 0.0 3 159 8 1 1 90 137 0.0 3 134 2 1 91 239 0.0 3 224 15 92 150 0.0 3 143 5 2 93 142 0.0 3 130 9 2 1 94 112 0.0 3 100 10 0 2 95 243 0.0 3 225 15 3 96 149 0.0 3 139 8 2 97 309 0.0 3 286 20 2 1 98 86 0.0 3 77 9 99 33 0.0 3 28 3 2 100 213 0.0 3 86 106 17 4