#@ 'correct' metaseqR by P.Moulos (2014):
samplename filename condition paired stranded
SCR10-E145-4wt /media/raid/tmp/fck/cer/SCR10-E145-4wt.bam WT single yes
SCR12-E145-6Het /media/raid/tmp/fck/cer/SCR12-E145-6Het.bam Het single yes
SCR13-E145-3wt /media/raid/tmp/fck/cer/SCR13-E145-3wt.bam WT single yes
SCR15-E145Het7 /media/raid/tmp/fck/cer/SCR15-E145Het7.bam Het single yes
#@
Dear Silvia,
to clarify what has been done up to now
I list all sample IDs in all sequencing runs we have made.
The IDs are unique. If there are any mistakes (e.g. for SCR21 and SCR22)
or missing information (e.g. 'P1' is missing in all IDs),
please edit this list
so we can sort the results.
BW,
Martin
run20
SC1_E15.5_1-3
SC2_E15.5_2-4
run106
SCR10-E145-4wt
SCR12-E145-6Het
run107
SCR13-E145-3wt
SCR14-E145-5Het
run118
SCR15-E145HET7 R_2014_12_02_12_33_41_user_ION-118-SCereghini_RNAseq_SCR15-ITlab-ChIP_ITC38-42-43_141202.SCR15.IonXpressRNA_016.fastq.gz
run127
SCR16r-65n2mut
SCR18r-65n8wt
DrSCereghini_4RNAseq_SCR16r-65n2mut-bc2_SCR18r-65n8wt-bc5
run128
SCR17r-65n5mut
SCR19r-65n11wt
run141
SCR21-7wt
SCR23-8Het
run143
SCR20-6wt
SCR22-5Het
run299 (3UTRseq)
SC3R1-1M14
SC3R2-3M14
SC3R3-6M14mut
SC3R4-8M14mut
SC3R5-9M14mut
SC3R6-1F16
SC3R7-6F16
SC3R8-2F16mut
SC3R9-5F16mut
SC3R10-8F16mut
/data/images/proton/run107/samples.txt
Barcode Name Sample Bases >=Q20 Bases Reads Mean Read Length
No barcode None 192705514 163397304 1823405 105 bp
IonXpressRNA_002 SCR13 6700709168 6013307709 72340797 92 bp
IonXpressRNA_010 SCR14 1675181557 1492520215 14801845 113 bp
DrSCereghini_4RNAseqsamples_SCR13-E145-3wt-bc2_SCR14-E145-5Het-bc10_SCR14 has been repurified 141104
/data/images/proton/run128/samples.txt
Barcode Name Sample Bases >=Q20 Bases Reads Mean Read Length
No barcode None 192809390 147479497 1452991 132 bp
IonXpressRNA_004 SCR17r 7280977907 5891911959 50831051 143 bp
IonXpressRNA_015 SCR19r 4957738983 4040853945 35550931 139 bp
DrSCereghini_4RNAseq_SCR17r-65n5mut-bc4_SCR19r-65n11wt-bc15
/data/images/proton/run141/samples.txt
Barcode Name Sample Bases >=Q20 Bases Reads Mean Read Length
No barcode None 203149442 148050060 2273612 89 bp
IonXpressRNA_005 SCR20-6wt 5291689056 4457739280 46440376 114 bp
IonXpressRNA_014 SCR22-5Het 3402202041 2937307254 36657973 93 bp
DrSCereghini_4RNAseq_SCR20-6wtt-bc5_SCR22-5Het-bc14
/data/images/proton/run143/samples.txt
Barcode Name Sample Bases >=Q20 Bases Reads Mean Read Length
No barcode None 135771582 101334477 1483666 92 bp
IonXpressRNA_009 SCR21-7wt 4539420375 3895138980 38446150 118 bp
IonXpressRNA_015 SCR23-8Het 6367014349 5334979798 47146473 135 bp
DrSCereghini_4RNAseq_SCR21-7wt-bc9_SCR23-8Het-bc15
run299
Read Length Histogram Files
No barcode None 978,168,943 765,971,089 8,254,988 118 bp UBAM BAM BAI
IonXpress_001 SC3R1-1M14 754,697,994 597,392,981 6,710,396 112 bp UBAM BAM BAI
IonXpress_002 SC3R2-3M14 527,791,920 418,455,318 4,694,087 112 bp UBAM BAM BAI
IonXpress_003 SC3R3-6M14mut 496,461,424 396,215,004 4,350,420 114 bp UBAM BAM BAI
IonXpress_004 SC3R4-8M14mut 432,029,510 345,246,424 3,935,188 110 bp UBAM BAM BAI
IonXpress_005 SC3R5-9M14mut 532,860,451 423,487,141 4,735,942 113 bp UBAM BAM BAI
IonXpress_006 SC3R6-1F16 427,300,477 335,264,669 3,658,897 117 bp UBAM BAM BAI
IonXpress_007 SC3R7-6F16 449,601,650 353,769,914 3,813,600 118 bp UBAM BAM BAI
IonXpress_008 SC3R8-2F16mut 630,829,776 500,084,022 5,693,795 111 bp UBAM BAM BAI
IonXpress_009 SC3R9-5F16mut 513,269,492 408,979,910 4,434,506 116 bp UBAM BAM BAI
IonXpress_010 SC3R10-8F16mut 525,157,166 412,182,876 4,954,907 106 bp UBAM BAM BAI
/data/images/proton/Cereghini/run142/metaseqr_wt_vs_mut_run142/index.html
Conditions: WT, HET
Samples: SCR20, SCR21, SCR22, SCR23
/data/images/proton/Cereghini/run142/metaseqr_wt_vs_het_run142_woSCR22/index.html
Conditions: WT, HET
Samples: SCR20, SCR21, SCR23
The negative log2(fold_change) in
Cereghini/Tuxedo_run143/gene_exp.diff
are cmoparable with the log2_normalized_fold_change_WT_vs_HET in
Cereghini/run142/metaseqr_wt_vs_het_run142_woSCR22/lists/metaseqr_sig_out_WT_vs_HET.txt.gz
/data/images/proton/Cereghini/run142/metaseqr_wt_vs_het_run142_woSCR22-scr21_wt_instead_of_het/index.html
Conditions: WT, HET
Samples: SCR20, SCR21, SCR23
/data/images/proton/run143/www/tuxedo/cuffdiff_HET_vs_WT/run.info
param value
cmd_line cuffdiff -L HET,WT --no-length-correction --library-type fr-secondstrand --no-update-check --num-threads 10 -q -o cuffdiff_HET_vs_WT merged_asm/merged.gtf RCAD_P1_HET_SCR23.bam2 RCAD_P1_WT_SCR20.bam2,RCAD_P1_WT_SCR21.bam2
version 2.2.1