#@ 'correct' metaseqR by P.Moulos (2014): samplename filename condition paired stranded SCR10-E145-4wt /media/raid/tmp/fck/cer/SCR10-E145-4wt.bam WT single yes SCR12-E145-6Het /media/raid/tmp/fck/cer/SCR12-E145-6Het.bam Het single yes SCR13-E145-3wt /media/raid/tmp/fck/cer/SCR13-E145-3wt.bam WT single yes SCR15-E145Het7 /media/raid/tmp/fck/cer/SCR15-E145Het7.bam Het single yes #@ Dear Silvia, to clarify what has been done up to now I list all sample IDs in all sequencing runs we have made. The IDs are unique. If there are any mistakes (e.g. for SCR21 and SCR22) or missing information (e.g. 'P1' is missing in all IDs), please edit this list so we can sort the results. BW, Martin run20 SC1_E15.5_1-3 SC2_E15.5_2-4 run106 SCR10-E145-4wt SCR12-E145-6Het run107 SCR13-E145-3wt SCR14-E145-5Het run118 SCR15-E145HET7 R_2014_12_02_12_33_41_user_ION-118-SCereghini_RNAseq_SCR15-ITlab-ChIP_ITC38-42-43_141202.SCR15.IonXpressRNA_016.fastq.gz run127 SCR16r-65n2mut SCR18r-65n8wt DrSCereghini_4RNAseq_SCR16r-65n2mut-bc2_SCR18r-65n8wt-bc5 run128 SCR17r-65n5mut SCR19r-65n11wt run141 SCR21-7wt SCR23-8Het run143 SCR20-6wt SCR22-5Het run299 (3UTRseq) SC3R1-1M14 SC3R2-3M14 SC3R3-6M14mut SC3R4-8M14mut SC3R5-9M14mut SC3R6-1F16 SC3R7-6F16 SC3R8-2F16mut SC3R9-5F16mut SC3R10-8F16mut /data/images/proton/run107/samples.txt Barcode Name Sample Bases >=Q20 Bases Reads Mean Read Length No barcode None 192705514 163397304 1823405 105 bp IonXpressRNA_002 SCR13 6700709168 6013307709 72340797 92 bp IonXpressRNA_010 SCR14 1675181557 1492520215 14801845 113 bp DrSCereghini_4RNAseqsamples_SCR13-E145-3wt-bc2_SCR14-E145-5Het-bc10_SCR14 has been repurified 141104 /data/images/proton/run128/samples.txt Barcode Name Sample Bases >=Q20 Bases Reads Mean Read Length No barcode None 192809390 147479497 1452991 132 bp IonXpressRNA_004 SCR17r 7280977907 5891911959 50831051 143 bp IonXpressRNA_015 SCR19r 4957738983 4040853945 35550931 139 bp DrSCereghini_4RNAseq_SCR17r-65n5mut-bc4_SCR19r-65n11wt-bc15 /data/images/proton/run141/samples.txt Barcode Name Sample Bases >=Q20 Bases Reads Mean Read Length No barcode None 203149442 148050060 2273612 89 bp IonXpressRNA_005 SCR20-6wt 5291689056 4457739280 46440376 114 bp IonXpressRNA_014 SCR22-5Het 3402202041 2937307254 36657973 93 bp DrSCereghini_4RNAseq_SCR20-6wtt-bc5_SCR22-5Het-bc14 /data/images/proton/run143/samples.txt Barcode Name Sample Bases >=Q20 Bases Reads Mean Read Length No barcode None 135771582 101334477 1483666 92 bp IonXpressRNA_009 SCR21-7wt 4539420375 3895138980 38446150 118 bp IonXpressRNA_015 SCR23-8Het 6367014349 5334979798 47146473 135 bp DrSCereghini_4RNAseq_SCR21-7wt-bc9_SCR23-8Het-bc15 run299 Read Length Histogram Files No barcode None 978,168,943 765,971,089 8,254,988 118 bp UBAM BAM BAI IonXpress_001 SC3R1-1M14 754,697,994 597,392,981 6,710,396 112 bp UBAM BAM BAI IonXpress_002 SC3R2-3M14 527,791,920 418,455,318 4,694,087 112 bp UBAM BAM BAI IonXpress_003 SC3R3-6M14mut 496,461,424 396,215,004 4,350,420 114 bp UBAM BAM BAI IonXpress_004 SC3R4-8M14mut 432,029,510 345,246,424 3,935,188 110 bp UBAM BAM BAI IonXpress_005 SC3R5-9M14mut 532,860,451 423,487,141 4,735,942 113 bp UBAM BAM BAI IonXpress_006 SC3R6-1F16 427,300,477 335,264,669 3,658,897 117 bp UBAM BAM BAI IonXpress_007 SC3R7-6F16 449,601,650 353,769,914 3,813,600 118 bp UBAM BAM BAI IonXpress_008 SC3R8-2F16mut 630,829,776 500,084,022 5,693,795 111 bp UBAM BAM BAI IonXpress_009 SC3R9-5F16mut 513,269,492 408,979,910 4,434,506 116 bp UBAM BAM BAI IonXpress_010 SC3R10-8F16mut 525,157,166 412,182,876 4,954,907 106 bp UBAM BAM BAI /data/images/proton/Cereghini/run142/metaseqr_wt_vs_mut_run142/index.html Conditions: WT, HET
Samples: SCR20, SCR21, SCR22, SCR23
/data/images/proton/Cereghini/run142/metaseqr_wt_vs_het_run142_woSCR22/index.html Conditions: WT, HET
Samples: SCR20, SCR21, SCR23
The negative log2(fold_change) in Cereghini/Tuxedo_run143/gene_exp.diff are cmoparable with the log2_normalized_fold_change_WT_vs_HET in Cereghini/run142/metaseqr_wt_vs_het_run142_woSCR22/lists/metaseqr_sig_out_WT_vs_HET.txt.gz /data/images/proton/Cereghini/run142/metaseqr_wt_vs_het_run142_woSCR22-scr21_wt_instead_of_het/index.html Conditions: WT, HET
Samples: SCR20, SCR21, SCR23
/data/images/proton/run143/www/tuxedo/cuffdiff_HET_vs_WT/run.info param value cmd_line cuffdiff -L HET,WT --no-length-correction --library-type fr-secondstrand --no-update-check --num-threads 10 -q -o cuffdiff_HET_vs_WT merged_asm/merged.gtf RCAD_P1_HET_SCR23.bam2 RCAD_P1_WT_SCR20.bam2,RCAD_P1_WT_SCR21.bam2 version 2.2.1